Insights into Human Astrocyte Response to H5N1 Infection by Microarray Analysis.
Identifieur interne : 000D25 ( Main/Exploration ); précédent : 000D24; suivant : 000D26Insights into Human Astrocyte Response to H5N1 Infection by Microarray Analysis.
Auteurs : Xian Lin [République populaire de Chine] ; Ruifang Wang [République populaire de Chine] ; Jun Zhang [République populaire de Chine] ; Xin Sun [République populaire de Chine] ; Zhong Zou [République populaire de Chine] ; Shengyu Wang [République populaire de Chine] ; Meilin Jin [République populaire de Chine]Source :
- Viruses [ 1999-4915 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Astrocytes.
- physiologie : Astrocytes, Sous-type H5N1 du virus de la grippe A.
- virologie : Astrocytes.
- Analyse de profil d'expression de gènes, Analyse sur microréseau, Animaux, Humains, Interactions hôte-pathogène, Stress physiologique.
English descriptors
- KwdEn :
- MESH :
- immunology : Astrocytes.
- physiology : Astrocytes, Influenza A Virus, H5N1 Subtype.
- virology : Astrocytes.
- Animals, Gene Expression Profiling, Host-Pathogen Interactions, Humans, Microarray Analysis, Stress, Physiological.
Abstract
Influenza virus infects not only the respiratory system but also the central nervous system (CNS), leading to influenza-associated encephalopathy and encephalitis. Astrocytes are essential for brain homeostasis and neuronal function. These cells can also be infected by influenza virus. However, genome-wide changes in response to influenza viral infection in astrocytes have not been defined. In this study, we performed gene profiling of human astrocytes in response to H5N1. Innate immune and pro-inflammatory responses were strongly activated at 24 h post-infection (hpi). Antiviral genes, as well as several cytokines and chemokines, including CXCL9, CXCL10, and CXCL11, were robustly induced. Phosphorylation of p65 and p38 can be activated by viral infection, suggesting their potential critical roles in H5N1-induced pro-inflammatory response. Moreover, H5N1 infection significantly upregulated the gene expressions related to the neuroactive ligand-receptor interaction pathway at 24 hpi, such as MC2R, CHRNG, P2RY13, GABRA1, and HRH2, which participant in synaptic transmission and may take part in CNS disorders induced by H5N1 infection. Targeting key components of innate immune response and the neuroactive ligand-receptor interaction pathway may provide a strategy to control H5N1-induced encephalopathy and encephalitis. This research can contribute to the understanding of H5N1 pathogenesis in astrocytes.
DOI: 10.3390/v7052618
PubMed: 26008703
Affiliations:
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yoya12@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Ruifang" sort="Wang, Ruifang" uniqKey="Wang R" first="Ruifang" last="Wang">Ruifang Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. wrf138493@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. zhangjun851029@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Sun, Xin" sort="Sun, Xin" uniqKey="Sun X" first="Xin" last="Sun">Xin Sun</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. coolxin@foxmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zou, Zhong" sort="Zou, Zhong" uniqKey="Zou Z" first="Zhong" last="Zou">Zhong Zou</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. zz19841024@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Shengyu" sort="Wang, Shengyu" uniqKey="Wang S" first="Shengyu" last="Wang">Shengyu Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. sywang2012@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Jin, Meilin" sort="Jin, Meilin" uniqKey="Jin M" first="Meilin" last="Jin">Meilin Jin</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. jinmeilin@mail.hzau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Astrocytes (immunology)</term><term>Astrocytes (physiology)</term><term>Astrocytes (virology)</term><term>Gene Expression Profiling</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Influenza A Virus, H5N1 Subtype (physiology)</term><term>Microarray Analysis</term><term>Stress, Physiological</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Analyse sur microréseau</term><term>Animaux</term><term>Astrocytes (immunologie)</term><term>Astrocytes (physiologie)</term><term>Astrocytes (virologie)</term><term>Humains</term><term>Interactions hôte-pathogène</term><term>Sous-type H5N1 du virus de la grippe A (physiologie)</term><term>Stress physiologique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Astrocytes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Astrocytes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Astrocytes</term><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Astrocytes</term><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Astrocytes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Astrocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Gene Expression Profiling</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Microarray Analysis</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Analyse sur microréseau</term><term>Animaux</term><term>Humains</term><term>Interactions hôte-pathogène</term><term>Stress physiologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza virus infects not only the respiratory system but also the central nervous system (CNS), leading to influenza-associated encephalopathy and encephalitis. Astrocytes are essential for brain homeostasis and neuronal function. These cells can also be infected by influenza virus. However, genome-wide changes in response to influenza viral infection in astrocytes have not been defined. In this study, we performed gene profiling of human astrocytes in response to H5N1. Innate immune and pro-inflammatory responses were strongly activated at 24 h post-infection (hpi). Antiviral genes, as well as several cytokines and chemokines, including CXCL9, CXCL10, and CXCL11, were robustly induced. Phosphorylation of p65 and p38 can be activated by viral infection, suggesting their potential critical roles in H5N1-induced pro-inflammatory response. Moreover, H5N1 infection significantly upregulated the gene expressions related to the neuroactive ligand-receptor interaction pathway at 24 hpi, such as MC2R, CHRNG, P2RY13, GABRA1, and HRH2, which participant in synaptic transmission and may take part in CNS disorders induced by H5N1 infection. Targeting key components of innate immune response and the neuroactive ligand-receptor interaction pathway may provide a strategy to control H5N1-induced encephalopathy and encephalitis. This research can contribute to the understanding of H5N1 pathogenesis in astrocytes.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Lin, Xian" sort="Lin, Xian" uniqKey="Lin X" first="Xian" last="Lin">Xian Lin</name></region><name sortKey="Jin, Meilin" sort="Jin, Meilin" uniqKey="Jin M" first="Meilin" last="Jin">Meilin Jin</name><name sortKey="Sun, Xin" sort="Sun, Xin" uniqKey="Sun X" first="Xin" last="Sun">Xin Sun</name><name sortKey="Wang, Ruifang" sort="Wang, Ruifang" uniqKey="Wang R" first="Ruifang" last="Wang">Ruifang Wang</name><name sortKey="Wang, Shengyu" sort="Wang, Shengyu" uniqKey="Wang S" first="Shengyu" last="Wang">Shengyu Wang</name><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name><name sortKey="Zou, Zhong" sort="Zou, Zhong" uniqKey="Zou Z" first="Zhong" last="Zou">Zhong Zou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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